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Thermo Fisher
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Thermo Fisher
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WITec gmbh
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1.5% crosslinking buffer (50 mm bacl 2 , 10 mm 3-(n-morpholino)propanesulfonic acid (mops), 140 mm d-mannitol, 0.025% vol/vol tween-20, ph 7.4) ![]() 1.5% Crosslinking Buffer (50 Mm Bacl 2 , 10 Mm 3 (N Morpholino)propanesulfonic Acid (Mops), 140 Mm D Mannitol, 0.025% Vol/Vol Tween 20, Ph 7.4), supplied by Nisco Engineering AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/1+mops+buffer/1+5++crosslinking+buffer++50+mm+bacl+2+++10+mm+3++n+morpholino+propanesulfonic+acid++mops+++140+mm+d+mannitol++0+025++vol+vol+tween+20++ph+7+4+/pmc06487074-100-27-38 Average 90 stars, based on 1 article reviews
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CPG Inc
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Image Search Results
Journal: Nature Communications
Article Title: Fluorescent sensors for imaging of interstitial calcium
doi: 10.1038/s41467-023-41928-w
Figure Lengend Snippet: Twelve combinations of export signal peptides and transmembrane/anchoring domains were used to target GreenT-EC to the surface of the cell. HeLa cells were transiently transfected with each construct and analyzed using flow cytometry. a Density plots of the negative control (non-transfected) indicating the gating strategy for selecting GreenT-EC positive single cells (indicated in slashed lines). Both the negative and the rest of the constructs displayed were resuspended in a MOPS buffer containing 3 mM CaCl 2 . b The fluorescence of GreenT-EC positive gated cells was further analyzed before and after the addition of EGTA (final concentration of 7.5 mM). Box-plots 90-10% are presented (whiskers are 90 and 10 percentiles, boxes are 75 and 25 percentiles and the center line correspond to the median). c Summary table compiling the main parameters of the experiment: Number of cells gated as GreenT-EC positive in each condition (n=cell count), mean fluorescence of GreenT-EC (mean fluoresce.), standard error of the mean (s.e.m) and the response calculated between the mean GreenT-EC fluorescence values before and after the addition of EGTA (ΔF/F 0 ). Source data are provided in the Source Data file.
Article Snippet: After centrifugation at 1000 g during 5 min, cells were resuspended in 1.5 ml
Techniques: Transfection, Construct, Flow Cytometry, Negative Control, Fluorescence, Concentration Assay, Cell Counting